ORYZALIN AS AN EFFICIENT AGENT FOR CHROMOSOME DOUBLING OF HAPLOID APPLE SHOOTS IN-VITRO

被引：43

作者：

BOUVIER, L ^{[1
]}

FILLON, FR ^{[1
]}

LESPINASSE, Y ^{[1
]}

机构：

[1] UNIV PICARDIE,F-80039 AMIENS,FRANCE

来源：

PLANT BREEDING | 1994年 / 113卷 / 04期

关键词：

MALUS X DOMESTICA; CHROMOSOME DOUBLING; COLCHICINE; ORYZALIN; IN VITRO CULTURE; HAPLOIDS;

D O I：

10.1111/j.1439-0523.1994.tb00748.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In an outbreeding species such as apple, haploid plants may be especially useful in breeding programmes for the production of homozygous material. However, methods must be available to induce chromosome doubling in the haploid plants. Two antimitotic agents, colchicine and oryzalin, were compared as regards their efficiency in inducing chromosome doubling of in vitro haploid apple shoots. Three colchicine levels (0.025, 0.25 and 1.25 mM) and three oryzalin levels (5, 15 and 30 mu M) were evaluated. Three techniques were also used and compared. Survival rate and chromosome counts were determined. Differences were observed between the two antimitotic agents and between the three techniques. This study demonstrates that oryzalin could be a better choice than colchicine for chromosome doubling on haploid apple shoots in vitro.

引用

页码：343 / 346

页数：4

共 13 条

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[2]

DERMEN H, 1945, AM J BOT, V22, P387

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