MICROTUBULAR ATPASE IN CARCINUS AND HELIX NERVE

1. 1. Fixed Carcinus leg nerves and Helix suboesophageal ganglia were incubated in a Wachstein-Meisel incubation medium to localize sites of ATPase activity. 2. 2. Lead phosphate precipitation indicating the location of an ATPase, was specifically deposited on mitochondrial inner and outer membranes, axonal membranes and on microtubules of both Carcinus and Helix nerves. 3. 3. The glutaraldehyde concentration affected the ATPase activity. 3% glutaraldehyde allowed location of the mitochondrial and axonal ATPase, whilst 1% glutaraldehyde allowed location of microtubular ATPase as well as the mitochondrial and axonal ATPase. © 1978.