MOLECULAR ANALYSIS OF THE BACTEROIDES-FRAGILIS-RECA GENE

被引：22

作者：

GOODMAN, HJK ^{[1
]}

WOODS, DR ^{[1
]}

机构：

[1] UNIV CAPE TOWN,DEPT MICROBIOL,RONDEBOSCH 7700,SOUTH AFRICA

来源：

GENE | 1990年 / 94卷 / 01期

关键词：

nucleotide sequence; protein homology; recA; Recombinant DNA; transcript mapping;

D O I：

10.1016/0378-1119(90)90470-C

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide sequence of a 2.5-kb DNA segment containing the Bacteroides fragilis recA gene was determined. The coding region of the recA gene specifies a protein of 318 amino acids. The RecA protein of B. fragilis shows significant homology with that of Escherichia coli, Thiobacillus ferooxidans, Pseudomonas aeruginosa and Proteus mirabilis. No SOS box characteristic of LexA-regulated promoters could be identified in the 5′-noncoding region of the B. fragilis recA gene. Promoter activity of the cloned recA gene in E. coli was located within a 113-bp fragment of the B. fragilis DNA by in vitro construction of operon fusions with a promoterless lacZ gene. The transcription start point for this gene in B. fragilis was determined by primer extension analysis. © 1990.

引用

页码：77 / 82

页数：6

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