THE AEROLYSIN MEMBRANE CHANNEL IS FORMED BY HEPTAMERIZATION OF THE MONOMER

被引：98

作者：

WILMSEN, HU

LEONARD, KR

TICHELAAR, W

BUCKLEY, JT

PATTUS, F

机构：

[1] EUROPEAN MOLEC BIOL LAB, MEYERHOFSTR 1, W-6900 HEIDELBERG, GERMANY

[2] UNIV VICTORIA, DEPT BIOCHEM & MICROBIOL, VICTORIA V8W 2Y2, BC, CANADA

来源：

EMBO JOURNAL | 1992年 / 11卷 / 07期

关键词：

AEROLYSIN; ELECTRON MICROSCOPY; MEMBRANE CHANNEL; TOXIN;

D O I：

10.1002/j.1460-2075.1992.tb05310.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytolytic toxin aerolysin has been found to form heptameric oligomers by SDS-PAGE electrophoresis, STEM mass measurements of single oligomers and image analysis of two-dimensional membrane crystals. Two types of crystal, flat sheets and long regular tubes, have been obtained by reconstitution of purified protein and Escherichia coli phospholipids. A noise-filtered image of the best crystalline sheets reveals a structure with 7-fold symmetry containing a central strongly stain-excluding ring that encircles a dark stain-filled channel 17 angstrom in diameter. The ring is surrounded by seven arms each made up of two unequal sized domains. By combining projected views and side-views, a simplified model of the aerolysin channel complex has been constructed. The relevance of this structure to the mode of action of aerolysin is discussed.

引用

页码：2457 / 2463

页数：7

共 22 条

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