STABLE TRANSFORMATION OF BARLEY CALLUS USING BIOLISTIC(R) PARTICLE BOMBARDMENT AND THE PHOSPHINOTHRICIN ACETYLTRANSFERASE (BAR) GENE

被引：7

作者：

STIFF, CM ^{[1
]}

KILIAN, A ^{[1
]}

ZHOU, HP ^{[1
]}

KUDRNA, DA ^{[1
]}

KLEINHOFS, A ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV, DEPT CROP & SOIL SCI, PULLMAN, WA 99164 USA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 40卷 / 03期

关键词：

ACCELERATED PARTICLE; HORDEUM VULGARE L; INTEGRATION; METHYLATION; SUSPENSION CULTURE;

D O I：

10.1007/BF00048130

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Suspension culture cells of barley (Hordeum vulgare L. cv. Klages) were collected on filters and bombarded with gold particles carrying the Basta (R) resistance (bar) gene, or both bar and the beta-glucuronidase (uidA) genes in a Biolistic (R) Particle Accelerator. Filters carrying the bombarded tissues were selected on basal medium with increasing concentrations (maximum of 200 mg 1(-1)) of phosphinothricin. Surviving calli were assayed for the presence of phosphinothricin acetyltransferase activity. All positive calli were shown to have the bar gene integrated into their genome. Nine independent stable transformation events have been analyzed with substantial variation in the integration pattern and transgene copy number. Some albino plantlets were produced, but no mature plants were obtained.

引用

页码：243 / 248

页数：6

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