COMPARATIVE-STUDY OF NONPOROUS ANION-EXCHANGE CHROMATOGRAPHY, CAPILLARY GEL-ELECTROPHORESIS AND CAPILLARY ELECTROPHORESIS IN POLYMER-SOLUTIONS IN THE SEPARATION OF DNA RESTRICTION FRAGMENTS

被引：29

作者：

SUMITA, C ^{[1
]}

BABA, Y ^{[1
]}

HIDE, K ^{[1
]}

ISHIMARU, N ^{[1
]}

SAMATA, K ^{[1
]}

TANAKA, A ^{[1
]}

TSUHAKO, M ^{[1
]}

机构：

[1] KOBE WOMENS COLL PHARM,HIGASHINADA KU,KOBE 658,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 661卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)85197-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

HPLC using a 100-mm column packed with a non-porous anion exchanger gave high-resolution separations of DNA restriction fragments up to 8000 base pairs (bp). High-resolution separation of DNA restriction fragments up to 12 000 bp was achieved using capillary gel electrophoresis (CGE) and capillary electrophoresis (CE) in entangled polymer solutions. These methods were compared with respect to their performance and efficiencies in the resolution of DNA restriction fragments. The resolving power of CGE is higher than those of the other techniques. The 50-100 bp resolution of DNA fragments up to 500 bp was realized using HPLC, 50 bp resolution using CE in entangled polymer solutions and 10 bp resolution using CGE. The plate number that was achieved with CGE of 3.10(6) plates/m was higher than those of HPLC of 1.10(6) and CE in polymer solutions of 7.10(5) plates/m. The advantages and limitations of HPLC and capillary electrophoretic techniques are discussed.

引用

页码：297 / 303

页数：7

共 20 条

[1] OPTIMIZATION OF GRADIENTS IN ANION-EXCHANGE SEPARATIONS OF OLIGONUCLEOTIDES USING COMPUTER-ASSISTED RETENTION PREDICTION AND A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SIMULATION SYSTEM [J].