PRODUCTION OF FERTILE TRANSGENIC MAIZE BY ELECTROPORATION OF SUSPENSION-CULTURE CELLS

被引：62

作者：

LAURSEN, CM ^{[1
]}

KRZYZEK, RA ^{[1
]}

FLICK, CE ^{[1
]}

ANDERSON, PC ^{[1
]}

SPENCER, TM ^{[1
]}

机构：

[1] DISCOVERY RES,DEKALB PLANT GENET,MYSTIC,CT 06355

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 24卷 / 01期

关键词：

ZEA MAYS L; TRANSFORMATION; ELECTROPORATION; BAR; PHOSPHINOTHRICIN ACETYLTRANSFERASE;

D O I：

10.1007/BF00040573

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid containing the bar gene, resulted in high-frequency recovery of stably transformed callus lines. Plants were regenerated from thirteen transformed callus lines and transmission of bar to progeny was demonstrated.

引用

页码：51 / 61

页数：11

共 26 条

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