ENHANCED ALBUMIN PROTEIN SEPARATIONS AND PROTEIN DRUG-BINDING CONSTANT MEASUREMENTS USING ANTIINFLAMMATORY DRUGS AS RUN BUFFER ADDITIVES IN AFFINITY CAPILLARY ELECTROPHORESIS

被引：40

作者：

SUN, P ^{[1
]}

HOOPS, A ^{[1
]}

HARTWICK, RA ^{[1
]}

机构：

[1] SUNY BINGHAMTON, DEPT CHEM, BINGHAMTON, NY 13902 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1994年 / 661卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00372-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An affinity capillary electrophoresis (ACE) method for improving albumin protein separations has been developed. Separation efficiencies for bovine serum albumin (BSA) and human serum albumin (HSA) are dramatically improved by using anti-inflammatory compounds as run buffer additives. The anti-inflammatory drugs used as biospecific ligands to improve the protein separation include ibuprofen (IB), flurbiprofen (FL), and ketoprofen (KE). The binding constants of proteins (BSA and HSA) for the anti-inflammatory ligands (FL and IB) are estimated by ACE and compared to literature values.

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页码：335 / 340

页数：6

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