EFFICIENT PRODUCTION OF CHICKEN EGG-YOLK ANTIBODIES AGAINST A CONSERVED MAMMALIAN PROTEIN

被引：309

作者：

GASSMANN, M ^{[1
]}

THOMMES, P ^{[1
]}

WEISER, T ^{[1
]}

HUBSCHER, U ^{[1
]}

机构：

[1] UNIV ZURICH, DEPT PHARMACOL & BIOCHEM, CH-8057 ZURICH, SWITZERLAND

来源：

FASEB JOURNAL | 1990年 / 4卷 / 08期

关键词：

Chicken antibodies; Conserved protein; Egg yolk; IgY; Proliferating cell nuclear antigen;

D O I：

10.1096/fasebj.4.8.1970792

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The egg yolk of immunized chicken is a rich and inexpensive source of specific polyclonal antibodies. In this paper we show that 20-30 μg of a highly conserved mammalian protein, as exemplified by proliferating cell nuclear antigen, are sufficient to induce an immune response. Immunoblot analysis revealed that specific antibodies appeared 20 days after immunization, reached a plateau after 30 days, and remained high until at least day 81. A total amount of 4 g immunoglobulin was extracted from 62 eggs of one immunized hen, yielding approximately 130 mg of specific antibodies.

引用

页码：2528 / 2532

页数：5

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