INCREASED EXPRESSION INVIVO AND INVITRO OF FOREIGN GENES DIRECTED BY A-TYPE INCLUSION BODY HYBRID PROMOTERS IN RECOMBINANT VACCINIA VIRUSES

被引：23

作者：

FUNAHASHI, SI

ITAMURA, S

IINUMA, H

NEROME, K

SUGIMOTO, M

SHIDA, H

机构：

[1] KYOTO UNIV,INST VIRUS RES,SAKYO KU,KYOTO 606,JAPAN

[2] TONEN CORP,CORP RES & DEV LAB,SAITAMA 354,JAPAN

[3] NATL INST HLTH,DEPT VIROL & RICKETTSIOL,SHINAGAWA KU,TOKYO 141,JAPAN

[4] TEIKYO UNIV,SCH MED,DEPT SURG 2,ITABASHI KU,TOKYO 173,JAPAN

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 10期

关键词：

D O I：

10.1128/JVI.65.10.5584-5588.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We constructed A-type inclusion body (ATI) hybrid promoters, that is, late ATI promoters followed by tandemly repeated early regions of the promoter for the 7.5-kDa protein (the 7.5-kDa promoter). The repetition of the whole early promoter sequence of the 7.5-kDa gene, including the upstream consensus sequence and initiation region, efficiently increased the early expression of the bacterial chloramphenicol acetyltransferase gene in recombinant vaccinia virus. Recombinant vaccinia virus could express influenza virus hemagglutinin via the hybrid promoter more efficiently, induced higher levels of neutralizing antibody and cytotoxic T lymphocytes, and consequently protected mice more efficiently against challenge with influenza virus than did recombinant vaccinia virus containing the widely used 7.5-kDa promoter.

引用

页码：5584 / 5588

页数：5

共 26 条

[1] EXPRESSION OF HERPES-SIMPLEX VIRUS-1 GLYCOPROTEIN-B BY A RECOMBINANT VACCINIA VIRUS AND PROTECTION OF MICE AGAINST LETHAL HERPES-SIMPLEX VIRUS-1 INFECTION [J].