THERMOSTABLE, SALT-TOLERANT AMYLASE FROM BACILLUS-SP-64

被引：16

作者：

KHIRE, JM

PANT, A

机构：

[1] Division of Biochemical Sciences, National Chemical Laboratory, Pune

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 02期

关键词：

BACILLUS; SALT-TOLERANT AMYLASE; THERMOSTABLE AMYLASE;

D O I：

10.1007/BF01195840

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A thermostable, salt-tolerant amylase was produced by Bacillus sp. 64, with maximum amylase production (8.0 U/ml culture filtrate) after 24-h growth. Partially purified amylase was stable at 60-degrees-C for 30 min and 80% of the original activity was retained when incubated in 5 m NaCl over 24 h. Starch or dextrin was the best carbon source and peptone the best nitrogen source for the production of the enzyme. Amylase was secreted over a wide pH range (5 to 11) with the maximum activity between pH 7 and 8. Ca2+ and Mg2+ stimulated growth and enzyme production.

引用

页码：167 / 170

页数：4

共 8 条

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