MUSCARINIC CHOLINERGIC RECEPTORS ON MURINE LYMPHOCYTE SUBPOPULATIONS - SELECTIVE INTERACTIONS WITH 2ND MESSENGER RESPONSE SYSTEM UPON PHARMACOLOGICAL STIMULATION

被引:15
作者
GENARO, AM [1 ]
CREMASCHI, GA [1 ]
BORDA, ES [1 ]
机构
[1] CONSEJO NACL INVEST CIENT & TECN,CEFYBO,SERRANO 665,RA-1414 BUENOS AIRES,ARGENTINA
来源
IMMUNOPHARMACOLOGY | 1993年 / 26卷 / 01期
关键词
LYMPHOCYTE; CHOLINERGIC RECEPTOR; CGMP; CAMP; PHOSPHOINOSITIDE TURNOVER;
D O I
10.1016/0162-3109(93)90063-V
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In this study the muscarinic cholinergic receptor capacity and affinity in murine lymphocyte sets and subsets and the biochemical responses obtained by the pharmacological stimulation of cholinergic receptors in these cells was determined by means of binding studies. Saturation assays with the specific radioligand for muscarinic cholinergic receptors ([H-3]QNB) showed that B lymphocytes lack high affinity muscarinic cholinergic receptors, while the binding on T cells was a specific and saturable process. Lyt 2.2+ cells have a significantly higher number of receptors (B(max)) than L3T4+ cells; but the equilibrium dissociation constant (K(d)) values obtained in both subpopulations did not differ significantly from one another, and resembled the K(d) Value Of T lymphocyte populations. The specific receptor stimulation by carbachol caused a different intracellular signal transduction according the tested cell subtypes. The muscarinic cholinergic stimulation result in a significant inhibition of isoproterenol-stimulated adenylate cyclase system in T, L3T4+ and Lyt 2.2+ cells. On the contrary, Lyt 2.2+ cells were only able to respond to carbachol stimulus increasing cGMP levels and inositol phosphate formation while L3T4+ cells were unable to do it. Results show differences in the expression and in the ability of cholinergic receptors in sets and subsets of murine lymphocytes to trigger intracellular second-messenger systems. The differential receptor expression and the second-messenger response systems could be important to study the modulation of cellular immune response by cholinergic stimulation.
引用
收藏
页码:21 / 29
页数:9
相关论文
共 34 条
[1]   A CHOLINERGIC RECEPTOR-SITE ON MURINE LYMPHOCYTES WITH NOVEL BINDING CHARACTERISTICS [J].
ATWEH, SF ;
GRAYHACK, JJ ;
RICHMAN, DP .
LIFE SCIENCES, 1984, 35 (24) :2459-2469
[2]   PHARMACOLOGICAL DIFFERENCES BETWEEN MUSCARINIC RECEPTORS COUPLED TO PHOSPHOINOSITIDE TURNOVER AND THOSE COUPLED TO ADENYLATE-CYCLASE INHIBITION [J].
BAUMGOLD, J ;
WHITE, T .
BIOCHEMICAL PHARMACOLOGY, 1989, 38 (10) :1605-1616
[3]   MUSCARINIC CHOLINERGIC RECEPTORS ON INTACT HUMAN-LYMPHOCYTES PROPERTIES AND SUBCLASS CHARACTERIZATION [J].
BERING, B ;
MOISES, HW ;
MULLER, WE .
BIOLOGICAL PSYCHIATRY, 1987, 22 (12) :1451-1458
[4]   LITHIUM AMPLIFIES AGONIST-DEPENDENT PHOSPHATIDYLINOSITOL RESPONSES IN BRAIN AND SALIVARY-GLANDS [J].
BERRIDGE, MJ ;
DOWNES, CP ;
HANLEY, MR .
BIOCHEMICAL JOURNAL, 1982, 206 (03) :587-595
[5]  
BROWN JH, 1984, FED PROC, V43, P2613
[6]  
BROWN JH, 1986, MOL PHARMACOL, V30, P566
[7]  
BULLOCH K, 1985, NEURAL MODULATION IM, P111
[8]   MUSCARINIC CHOLINERGIC BINDING-SITES ON RAT LYMPHOCYTES [J].
COSTA, LG ;
KAYLOR, G ;
MURPHY, SD .
IMMUNOPHARMACOLOGY, 1988, 16 (03) :139-149
[9]  
DEGEORGE JJ, 1987, J BIOL CHEM, V262, P8077
[10]  
DOODS HN, 1987, J PHARMACOL EXP THER, V242, P257