EFFECT OF FRUSEMIDE, ETHACRYNIC-ACID AND INDANYLOXYACETIC ACID ON SPONTANEOUS CA-ACTIVATED CURRENTS IN RABBIT PORTAL-VEIN SMOOTH-MUSCLE CELLS

1 The effect of frusemide, ethacrynic acid and indanyloxyacetic acid was investigated on spontaneous calcium-activated chloride (I-Cl(Ca)) and potassium currents (I-K(Ca)) in rabbit portal vein cells with the perforated patch technique. 2 Frusemide (0.3-1.0 x 10(-3) M) reduced the amplitude of spontaneous transient inward chloride currents (STICs) in a concentration-dependent manner. The degree of inhibition on STIC amplitude was similar between -50 and +30 mV and frusemide did not alter the STIC reversal potential (E(rev)). 3 The voltage-dependent exponential decay of STICs, which is thought to represent closure bf chloride channels, was not altered by frusemide. 4 The amplitude and frequency of spontaneous potassium outward currents (STOCs) were not altered by frusemide. Since both STICs and STOCs are activated by calcium released from intracellular stores these data indicate that frusemide may block directly I-Cl(Ca). 5 Ethacrynic acid (2-5 x 10(-4) M) decreased the amplitude of STICs in a concentration-dependent manner by a similar amount at potentials of -50 to +30 mV but did not alter the STIC E(rev). However, these concentrations of ethacrynic acid also reduced STOC amplitude and 5 x 10(-4) M ethacrynic acid evoked a sustained outward current in most cells at 0 mV; thus ethacrynic acid has a more complex action than simple block of I-Cl(Ca). 6 Indanyloxyacetic acid reduced both STIC amplitude and decay time without affecting STOCs and thus also seems to inhibit directly I-Cl(Ca). It is discussed whether block of I-Cl(Ca) mediates the vasodilator effect of these agents.