ASSESSING SUCCESS IN GENE-TRANSFER BETWEEN LOLIUM-MULTIFLORUM AND FESTUCA-ARUNDINACEA

被引:19
作者
HUMPHREYS, MW [1 ]
GHESQUIERE, M [1 ]
机构
[1] INRA,AMELIORAT PLANTES FOURRAGERES STN,CTR POITOU CHARENTES,F-86600 LUSIGNAN,FRANCE
关键词
D O I
10.1007/BF02262643
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A Festuca arundinacea (2n = 6x = 42) plant with three PGI/2 homoeoalleles marking three homoeologous chromosomes was crossed with a Lolium multiflorum plant (2n = 4x = 28) with a different PGI/2 phenotype to give a pentaploid hybrid (2n = 5x = 35) with five chromosomes each marked by a different PGI/2 allele. This hybrid plant was backcrossed twice with diploid L. multiflorum (2n = 2x = 14) with a different PGI/2 phenotype. Numbers of interspecific recombinants involving chromosomes marked by PGI/2 were then determined in both backcross generations. In the BCl, recombinants involving only one PGI/2 allele were found but in the BC2, all three homoeologous F. arundinacea chromosomes carrying the PGI/2 locus recombined with Lolium with one in greater frequency and the others in equal but lower frequency. The evidence supports claims made for F. pratensis (2n = 2x = 14) and F. arundinacea var. glaucescens (2n = 4x = 28) being progenitors for F. arundinacea.
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页码:283 / 289
页数:7
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