SPATIAL AND TEMPORAL CA2+ SIGNALING IN ARTICULAR CHONDROCYTES

被引:20
作者
DANDREA, P
VITTUR, F
机构
[1] Univ Trieste, Dipartimento Biochim Biofis and Chim Macromolec, I 34127 Trieste
关键词
D O I
10.1006/bbrc.1995.2442
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stimulation of pig articular chondrocytes with either bradykinin, fetal calf serum or the Ca2+-ATPase inhibitor thapsigargin induced increases of the cytosolic Ca2+ concentration. By computerized videoimaging, the spatial and temporal aspects of the Ca2+ signal were revealed at single cell level. The cell response depended on Ca2+ release from intracellular stores without significant contribution of Ca2+ influx. A response initiated in a discrete subcellular region and eat heterogeneity in the cell population was found with respect to the Ca2+ storage ability. The Ca2+ response initiated in a discrete subcellular region and then spread in a nondecremental fashion to involve the whole cytosol. Such a behaviour was independent of the stimulus applied, thus suggesting a functional heterogeneity of the intracellular Ca2+ stores involved. In the region from which the response started, local Ca2+ spikes were recorded, revealing a spatially restricted pulsatile activity. (C) 1995 Academic Press,Inc.
引用
收藏
页码:129 / 135
页数:7
相关论文
共 14 条
[1]  
ALBRITTON NL, 1993, CELL CALCIUM, V14, P691
[2]   IDENTIFICATION OF A NOVEL INFLAMMATORY STIMULANT OF CHONDROCYTES - EARLY EVENTS IN CELL ACTIVATION BY BRADYKININ RECEPTORS ON PIG ARTICULAR CHONDROCYTES [J].
BENTON, HP ;
JACKSON, TR ;
HANLEY, MR .
BIOCHEMICAL JOURNAL, 1989, 258 (03) :861-867
[3]   IDENTIFICATION OF A COMMON CLASS OF HIGH-AFFINITY RECEPTORS FOR BOTH TYPES OF PORCINE INTERLEUKIN-1 ON CONNECTIVE-TISSUE CELLS [J].
BIRD, TA ;
SAKLATVALA, J .
NATURE, 1986, 324 (6094) :263-266
[4]  
DANDREA P, 1993, J BIOL CHEM, V268, P15213
[5]   INOSITOL PHOSPHATES AND CA2+ ENTRY - TOWARD A PROLIFERATION OR A SIMPLIFICATION [J].
IRVINE, RF .
FASEB JOURNAL, 1992, 6 (12) :3085-3091
[6]   A NOVEL TUMOR PROMOTER, THAPSIGARGIN, TRANSIENTLY INCREASES CYTOPLASMIC FREE CA-2+ WITHOUT GENERATION OF INOSITOL PHOSPHATES IN NG115-401L NEURONAL CELLS [J].
JACKSON, TR ;
PATTERSON, SI ;
THASTRUP, O ;
HANLEY, MR .
BIOCHEMICAL JOURNAL, 1988, 253 (01) :81-86
[7]   AGONIST-STIMULATED DIVALENT-CATION ENTRY INTO SINGLE CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL-CELLS [J].
JACOB, R .
JOURNAL OF PHYSIOLOGY-LONDON, 1990, 421 :55-77
[8]   SUBCELLULAR-DISTRIBUTION OF CA2+ RELEASE CHANNELS UNDERLYING CA2+ WAVES AND OSCILLATIONS IN EXOCRINE PANCREAS [J].
KASAI, H ;
LI, YX ;
MIYASHITA, Y .
CELL, 1993, 74 (04) :669-677
[9]  
MALEMUD CJ, 1992, BIOL REGULATION CHON, P295
[10]  
NEWTON CL, 1994, J BIOL CHEM, V269, P28613