NEW RIBOSOMAL-PROTEIN LOCUS IN ESCHERICHIA-COLI - GENE FOR PROTEIN S6 MAPS AT 97 MIN

A mutant of E. coli selected for temperature-sensitive growth on rich medium harbored an altered ribosomal protein S6. This mutant possessed at least 2 mutations, 1 being responsible for the temperature-sensitivity and the other for the S6 alteration. Crosses with various Hfr strains and transductions with [phage] P1kc revealed that the former mutation mapped at 98 min and the latter at 97 min. Furthermore, recA derivatives of this mutant heteromerodiploid for this region possessed the wild type and the mutant forms of S6. Thus, the gene at 97 min was indeed the structural gene for protein S6 (rpsF) and not a gene modifying it.