PURIFICATION AND CHARACTERIZATION OF A BETA-GLUCOSIDASE FROM STREPTOMYCES-LIVIDANS 66

被引：21

作者：

MIHOC, A

KLUEPFEL, D

机构：

[1] UNIV QUEBEC,INST ARMAND FRAPPIER,CTR RECH MICROBIOL APPL,531 BLVD PRAIRIES,LAVAL H7N 4Z3,QUEBEC,CANADA

[2] NATL RES COUNCIL CANADA,INST BIOTECHNOL,MONTREAL H4P 2R2,QUEBEC,CANADA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1990年 / 36卷 / 01期

关键词：

Characterization; Purification; Streptomyces lividans; β-glucosidase;

D O I：

10.1139/m90-009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An intracellular β-1,4-D-glucosidase (EC 3.2.1.21) was isolated from the mutant strain HP-3 of Streptomyces lividans 66 which produced about 12 times more enzyme than the wild-type strain. The purification was carried out by anion exchange column chromatography followed by high-performance liquid chromatography on DEAE and on molecular sieve columns. The enzyme is glycosylated and has an apparent M(r) of 51,000 and a pI of 4.3. Its activity was optimal at pH 6.5 and at a temperature of 40°C. The K(m) and the V(max) on cellobiose were 3.1 mM an 65.6 μmol min-1 mg-1 of enzyme.

引用

页码：53 / 56

页数：4

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[3]

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