RECONSTITUTION OF ACTIVE 30-S RIBOSOMAL-SUBUNITS INVITRO USING HEAT-DENATURED 16-S RIBOSOMAL-RNA

被引：7

作者：

BARRITAULT, D ^{[1
]}

GUERIN, MF ^{[1
]}

HAYES, DH ^{[1
]}

机构：

[1] INST BIOL PHYS CHIM,CHIM CELLULAIRE LAB,F-75005 PARIS,FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13217.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

30‐S ribosomal subunits which have been reconstituted using heat‐denatured 16‐S rRNA can participate in the synthesis of lysosyme in vitro. Therefore all the information contributed by 16‐S rRNA to the reconstitution process is carried in the primary sequence of this RNA. The specific protein‐synthesizing activity of 30‐S subunits reconstituted from 30‐S subunit proteins and heat‐denatured 16‐S rRNA is about one third of that observed if unheated 16‐S rRNA is used and is comparable to the activity of 30‐S particles isolated after dissociation of 70‐S ribosomes in the presence of 0.1 mM Mg2+. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：567 / 571

页数：5

共 25 条

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