INTERACTION OF HUMAN PLASMA FIBRONECTIN WITH ALPHA-ELASTIN

被引:9
作者
HARUMIYA, S
JUNG, SK
SAKANO, Y
FUJIMOTO, D
机构
[1] Laboratory of Biological Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu
关键词
D O I
10.1093/oxfordjournals.jbchem.a124108
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Because there are contradictory reports about the interaction of plasma fibronectin with elastin, we investigated the interaction in vitro. When human plasma was applied to an alpha-elastin-Sepharose column at 4-degrees-C, the column-binding fraction contained fibronectin. When isolated plasma fibronectin was applied to the same column at 4-degrees-C, most of the fibronectin bound to the column and was eluted with 1 M KBr. However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25-degrees-C than at 4-degrees-C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. The elevation of the surface hydrophobicity of plasma fibronectin at 4-degrees-C was observed by hydrophobic chromatography, using alkyl-Sepharose columns. It seems that the binding of plasma fibronectin to alpha-elastin involves hydrophobic interaction, which is affected by temperature and possibly by other factors.
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页码:710 / 714
页数:5
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