APPLICATION OF BACTERIAL MAGNETIC PARTICLES AS NOVEL DNA CARRIERS FOR BALLISTIC TRANSFORMATION OF A MARINE CYANOBACTERIUM

被引：49

作者：

TAKEYAMA, H ^{[1
]}

YAMAZAWA, A ^{[1
]}

NAKAMURA, C ^{[1
]}

MATSUNAGA, T ^{[1
]}

机构：

[1] TOKYO UNIV AGR & TECHNOL, DEPT BIOTECHNOL, KOGANEI, TOKYO 184, JAPAN

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 05期

关键词：

D O I：

10.1007/BF00638870

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

引用

页码：355 / 360

页数：6

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