THE ROLE OF THE CCAAT ENHANCER-BINDING PROTEIN IN THE TRANSCRIPTIONAL REGULATION OF THE GENE FOR PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP)

被引：210

作者：

PARK, EA ^{[1
]}

ROESLER, WJ ^{[1
]}

LIU, J ^{[1
]}

KLEMM, DJ ^{[1
]}

GURNEY, AL ^{[1
]}

THATCHER, JD ^{[1
]}

SHUMAN, J ^{[1
]}

FRIEDMAN, A ^{[1
]}

HANSON, RW ^{[1
]}

机构：

[1] CARNEGIE INST WASHINGTON,DEPT EMBRYOL,HOWARD HUGHES RES LABS,BALTIMORE,MD 21210

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 12期

关键词：

D O I：

10.1128/MCB.10.12.6264

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have identified a region in the promoter of the gene for phosphoenolpyruvate carboxykinase (GTP) (PEPCK) (positions -460 to +73) containing the regulatory elements which respond to cyclic AMP, glucocorticoids, and insulin and confer the tissue- and developmental stage-specific properties to the gene. We report that CCAAT/enhancer-binding protein (C/EBP) binds to the cyclic AMP-responsive element CRE-1 as well as to two regions which have been previously shown to bind proteins enriched in liver nuclei. The DNase I footprint pattern provided by the recombinant C/EBP was identical to that produced by a 43-k Da protein purified from rat liver nuclear extracts, using a CRE oligonucleotide affinity column, which was originally thought to be the CRE-binding protein CREB. Transient cotransfection experiments using a C/EBP expression vector demonstrated that C/EBP could trans activate the PEPCK promoter. The trans activation occurred through both the upstream, liver-specific protein-binding domains and the CRE. The CRE-binding protein bound only to CRE-1 and not to the upstream C/EBP-binding sites. The results of this study, along with physiological properties of C/EBP, indicate an important role for this transcription factor in providing the PEPCK gene with several of its regulatory characteristics.

引用

页码：6264 / 6272

页数：9

共 34 条

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