DETECTION OF EPSTEIN-BARR-VIRUS GENOMES IN AIDS RELATED LYMPHOMAS - SENSITIVITY AND SPECIFICITY OF INSITU HYBRIDIZATION COMPARED WITH SOUTHERN BLOTTING

被引:29
作者
HAMILTONDUTOIT, SJ
DELECLUSE, HJ
RAPHAEL, M
LENOIR, G
PALLESEN, G
机构
[1] INT AGCY RES CANC,F-69372 LYONS,FRANCE
[2] HOP PITIE,DEPT HAEMATOL,F-75651 PARIS 13,FRANCE
关键词
D O I
10.1136/jcp.44.8.676
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Eighteen cases of AIDS related, non-Hodgkin's lymphomas were examined for the presence of Epstein-Barr virus (EBV) genomes using in situ hybridisation with a S-35-labelled probe. The results were compared with those obtained independently by Southern blot analysis with a P-32-labelled probe of frozen tissue from the same tumours. Technically satisfactory results were obtained with both methods in 15 lymphomas. EBV DNA was detected in seven of 15 (47%) cases by in situ hybridisation and in eight of 15 (53%) cases by Southern blotting (including all the cases positive by in situ hybridisation). The results of EBV DNA detection by the two techniques were identical in 14 of 15 (93%) cases. In situ hybridisation gave no false positive results. This study shows that the sensitivity and specificity of in situ hybridisation for the detection of EBV genomes in AIDS related lymphomas approaches that of Southern blotting, even when using routinely processed archival, paraffin wax embedded material.
引用
收藏
页码:676 / 680
页数:5
相关论文
共 34 条
  • [1] BASHIR R, 1989, AM J PATHOL, V135, P1035
  • [2] DETECTION OF EPSTEIN-BARR VIRUS IN CNS LYMPHOMAS BY INSITU HYBRIDIZATION
    BASHIR, RM
    HARRIS, NL
    HOCHBERG, FH
    SINGER, RM
    [J]. NEUROLOGY, 1989, 39 (06) : 813 - 817
  • [3] DETECTION OF VIRAL GENOMES IN CULTURED-CELLS AND PARAFFIN-EMBEDDED TISSUE-SECTIONS USING BIOTIN-LABELED HYBRIDIZATION PROBES
    BRIGATI, DJ
    MYERSON, D
    LEARY, JJ
    SPALHOLZ, B
    TRAVIS, SZ
    FONG, CKY
    HSIUNG, GD
    WARD, DC
    [J]. VIROLOGY, 1983, 126 (01) : 32 - 50
  • [4] VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITTS LYMPHOMA
    EPSTEIN, MA
    ACHONG, BG
    BARR, YM
    [J]. LANCET, 1964, 1 (733) : 702 - +
  • [5] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY
    FEINBERG, AP
    VOGELSTEIN, B
    [J]. ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) : 6 - 13
  • [6] REPLICATION OF EPSTEIN-BARR VIRUS WITHIN THE EPITHELIAL-CELLS OF ORAL HAIRY LEUKOPLANKIA, AN AIDS-ASSOCIATED LESION
    GREENSPAN, JS
    GREENSPAN, D
    LENNETTE, ET
    ABRAMS, DI
    CONANT, MA
    PETERSEN, V
    FREESE, UK
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1985, 313 (25) : 1564 - 1571
  • [7] HAASE AT, 1984, METHOD VIROL, V7, P189
  • [8] HAMILTONDUTOIT SJ, 1990, MODERN PATHOLOGY OF AIDS AND OTHER RETROVIRAL INFECTIONS, P110
  • [9] HAMILTONDUTOIT SJ, 1989, LANCET, V1, P554
  • [10] HAMILTONDUTOIT SJ, 1991, AM J PATHOL, V138, P149