CALCIUM-DEPENDENT FLUORESCENCE LIFETIMES OF INDO-1 FOR ONE-PHOTON AND 2-PHOTON EXCITATION OF FLUORESCENCE

被引：45

作者：

SZMACINSKI, H ^{[1
]}

GRYCZYNSKI, I ^{[1
]}

LAKOWICZ, JR ^{[1
]}

机构：

[1] UNIV MARYLAND, CTR FLUORESCENCE SPECT, DEPT BIOL CHEM, 108 N GREENE ST, BALTIMORE, MD 21201 USA

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1993年 / 58卷 / 03期

关键词：

D O I：

10.1111/j.1751-1097.1993.tb09571.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We characterized the fluorescence intensity decays of Indo-1, which is commonly used as an emission wavelength-ratiometric calcium probe. The apparent lifetime of the long-wavelength side of the emission of Indo-1 is dependent on Ca2+. This long-wavelength emission displays the characteristics of an excited-state reaction, that is, a negative preexponential component in the multiexponential analysis. The emission spectra and lifetime of Indo-1 appear to be identical for one-photon and two-photon excitation at 351 and 702 mn, respectively, suggesting that the relative one- and two-photon cross sections are similar for the calcium-free and calcium-bound forms of Indo-1. Also, the two-photon cross section of Indo-I is relatively high, about 4 x 10(-49) cm4 s/photon molecule at 690 nm for both the calcium-free and calcium-bound forms. Hence, Indo-1 can be used for calcium imaging based on one- or two-photon excitation, using either emission wavelength ratios or lifetime imaging methods.

引用

页码：341 / 345

页数：5

共 35 条

[1]

AGARD DA, 1989, METHOD CELL BIOL, V30, P353

[2] ABSOLUTE 2-PHOTON ABSORPTIVITY OF TRANS-STILBENE NEAR 2-PHOTON ABSORPTION MAXIMUM VIA 3 WAVE MIXING [J].