LOCALIZATION OF AN EXCHANGEABLE GTP-BINDING SITE AT THE PLUS END OF MICROTUBULES

被引:147
作者
MITCHISON, TJ
机构
[1] Department of Pharmacology, University of California, San Francisco
关键词
D O I
10.1126/science.8102497
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Microtubule polarity arises from the head-to-tall orientation of alpha-beta tubulin heterodimers in the microtubule lattice. The identity of the polypeptide at each end of the microtubule is unknown, but structural models predict that the beta-tubulin end contains an exchangeable guanosine triphosphate (GTP) binding site. When GTP-coated fluorescent beads were incubated with microtubules, they bound specifically to plus ends, suggesting that tubulin is oriented in microtubules with beta-tubulin toward the plus end.
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页码:1044 / 1047
页数:4
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