IMMOBILIZATION OF YEAST-CELLS ONTO HYDROXYALKYL METHACRYLATE GELS MODIFIED BY SPACERS OF DIFFERENT LENGTHS

被引：6

作者：

GULAYA, VE

TURKOVA, J

JIRKU, V

FRYDRYCHOVA, A

COUPEK, J

ANANCHENKO, SN

机构：

[1] CZECHOSLOVAK ACAD SCI, INST ORGAN CHEM & BIOCHEM, CS-16610 PRAGUE 6, CZECHOSLOVAKIA

[2] MM SHEMYAKIN BIOORGAN CHEM INST, MOSCOW 117312, USSR

[3] LAB INSTRUMENTS WORKS, CS-16203 PRAGUE, CZECHOSLOVAKIA

[4] INST CHEM TECHNOL, DEPT FERMENTAT CHEM, CS-16628 PRAGUE, CZECHOSLOVAKIA

来源：

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1979年 / 8卷 / 1-2期

关键词：

D O I：

10.1007/BF00510265

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The potential of hydroxyalkyl methacrylate gel as a support for yeast cell immobilization by covalent attachment was investigated with respect to the length of spacer used and the manner of its activation. Saccharomyces paradoxus cells were immobilized successfully onto glutaraldehyde activated glycyl-, β-alanyl-and ε-aminocaproyl-derivatives of the above mentioned gel modified with hexamethylenediamine. Results suggest that covalent attachment via spacer is strongly influenced by cell surface characteristics. © 1979 Springer-Verlag.

引用

页码：43 / 47

页数：5

共 8 条

[1]

ANANCHENKO SN, 1963, TETRAHEDRON LETT, P1553

[2]

GIBIAN H, 1966, 2ND INT C HORM STER, P234

[3]

GULAYA V E, 1975, Prikladnaya Biokhimiya i Mikrobiologiya, V11, P657

[4]

GULAYA VE, 1979, BIOORG KHIM+, V5, P768

[5]

GULAYA VE, 1970, IZV AKAD NAUK, V8, P1811

[6]

JACK TR, 1977, ADV BIOCHEM ENG, V5, P143

[7] MODIFIED HYDROXYALKYL METHACRYLATE GEL AS A SUPPORT FOR IMMOBILIZATION OF YEAST-CELLS [J].

JIRKU, V ;

TURKOVA, J ;

KUCHYNKOVA, A ;

KRUMPHANZL, V .

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1979, 6 (03) :217-222

[8]

KOGAN LM, 1967, TETRAHEDRON LETT, P4673

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