THE COMBINATION OF AN AMPEROMETRIC INTEGRATED GLUCOSE SENSOR WITH FLOW-INJECTION ANALYSIS FOR BIOPROCESS CONTROL

被引:7
作者
BATAILLARD, P
HAEMMERLI, S
LUDI, H
WIDMER, HM
机构
[1] Central Analytical Research FO 3, Ciba - Geigy AG
关键词
D O I
10.1016/0925-4005(91)80128-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The on-line determination of glucose concentrations is of crucial importance for bioprocess control. Biosensors can offer decisive advantages, but only if they are treated as a component of a total analytical system. The glucose sensor we use is a miniaturized planar three-electrode structure with glucose oxidase immobilized on the electrode surface. The hydrogen peroxide produced by the enzymatic conversion of glucose is determined amperometrically. The performance of such a sensor is improved upon introduction into a flow injection analysis manifold, since this technique makes automatic calibration and sample preconditioning possible. Glucose is determined off-line and on-line during a yeast cultivation in conjunction with a reference method to compare data. However, matrix effects are still present, and signals due to interfering substances are significant at low glucose concentration levels. Different ways for eliminating these interferences and improving the stability of the enzymatic membrane are described.
引用
收藏
页码:309 / 313
页数:5
相关论文
共 11 条
[1]  
BROOKS SL, 1987, BIOSENSORS, V3, P45
[2]   A FLOW-INJECTION ANALYSIS SYSTEM FOR FERMENTATION MONITORING AND CONTROL [J].
GARN, M ;
GISIN, M ;
THOMMEN, C ;
CEVEY, P .
BIOTECHNOLOGY AND BIOENGINEERING, 1989, 34 (04) :423-428
[3]   FABRICATION AND CHARACTERIZATION OF A PLANAR ELECTROCHEMICAL-CELL AND ITS APPLICATION AS A GLUCOSE SENSOR [J].
GERNET, S ;
KOUDELKA, M ;
DEROOIJ, NF .
SENSORS AND ACTUATORS, 1989, 18 (01) :59-70
[4]   THE USE OF CHEMICAL SENSORS IN INDUSTRY [J].
GRABER, N ;
LUDI, H ;
WIDMER, HM .
SENSORS AND ACTUATORS B-CHEMICAL, 1990, 1 (1-6) :239-243
[5]   ENZYME ELECTRODE FOR AMPEROMETRIC DETERMINATION OF GLUCOSE [J].
GUILBAULT, GG ;
LUBRANO, GJ .
ANALYTICA CHIMICA ACTA, 1973, 64 (03) :439-455
[6]   CHARACTERIZATION OF PERFLUOROSULFONIC ACID POLYMER COATED ENZYME ELECTRODES AND A MINIATURIZED INTEGRATED POTENTIOSTAT FOR GLUCOSE ANALYSIS IN WHOLE-BLOOD [J].
HARRISON, DJ ;
TURNER, RFB ;
BALTES, HP .
ANALYTICAL CHEMISTRY, 1988, 60 (19) :2002-2007
[7]   PLANAR AMPEROMETRIC ENZYME-BASED GLUCOSE MICROELECTRODE [J].
KOUDELKA, M ;
GERNET, S ;
DEROOIJ, NF .
SENSORS AND ACTUATORS, 1989, 18 (02) :157-165
[8]   CONTROL OF MOLECULAR-WEIGHT SELECTIVITY IN ELECTRODE MODIFICATIONS BASED ON PHASE-INVERSION CELLULOSE-ACETATE MEMBRANES [J].
KUHN, LS ;
WEBER, SG ;
ISMAIL, KZ .
ANALYTICAL CHEMISTRY, 1989, 61 (04) :303-309
[9]   FLOW-INJECTION ANALYSIS AND BIOSENSORS - APPLICATIONS FOR BIOTECHNOLOGY AND ENVIRONMENTAL-CONTROL [J].
LUDI, H ;
GARN, MB ;
BATAILLARD, P ;
WIDMER, HM .
JOURNAL OF BIOTECHNOLOGY, 1990, 14 (01) :71-79
[10]  
PFEIFFER EF, 1988, HORMONE METABOLIC RE, V20