MOLECULAR-WEIGHT DETERMINATION OF UNDERIVATIZED OLIGODEOXYRIBONUCLEOTIDES BY POSITIVE-ION MATRIX-ASSISTED ULTRAVIOLET LASER-DESORPTION MASS-SPECTROMETRY

被引：98

作者：

SPENGLER, B ^{[1
]}

PAN, Y ^{[1
]}

COTTER, RJ ^{[1
]}

KAN, LS ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOLEC SCI,MIDDLE ATLANTIC MASS SPECTROMETRY FACIL,BALTIMORE,MD 21205

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 04期

关键词：

D O I：

10.1002/rcm.1290040402

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A Wiley‐McLaren type time‐of‐flight mass spectrometer has been used for molecular weight measurements of several unprotected oligodeoxyribonucleotides using matrix‐assisted UV laser desorption. Approximately 10 to 100 pmol of sample was required for recording their positive‐ion mass spectra with a mass resolution in the range of 150 to 300 (Full width at half maximum) (FWHM). Little fragmentation was observed. Copyright © 1990 Heyden & Son Limited

引用

页码：99 / 102

页数：4

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