IMMUNOLOGICAL MEASUREMENT OF POLYCYCLIC AROMATIC HYDROCARBON-ALBUMIN ADDUCTS IN FOUNDRY WORKERS AND ROOFERS

Roofers and iron foundry workers with high exposure to polycyclic aromatic hydrocarbons (PAH) were monitored for levels of covalent PAH serum albumin adducts, quantitated in enzymatically digested samples by a sensitive competitive enzyme-linked immunosorbent assay. Albumin adducts were higher in the foundry workers (5.22 fmol/mu-g, 0.314 mmol/mol) than in the reference group (4.07 fmol/mu-g, 0.245 mmol/mol), but only of borderline significance probably due to the small sample size. In a subset of foundry workers, a significant difference in adduct levels was observed for samples collected immediately after vacation and after six weeks of workplace exposure. The roofers also showed higher levels of adducts (5.19 fmol/mu-g, 0.312 mmol/mol) than their reference group (3.28 fmol/mu-g, 0.197 mmol/mol). These results demonstrate the feasibility of PAH protein adduct measurement as a marker of human exposure to this class of chemicals.