PLANT-REGENERATION FROM CALLUS-CULTURE OF 3 CITRUS ROOTSTOCKS

被引：11

作者：

BELOUALY, N

机构：

[1] Laboratoire de Cytogénétique, Université Catholique de Louvain, Louvain la Neuve, B 1348

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1991年 / 24卷 / 01期

关键词：

ADVENTITIOUS SHOOTS; CARRIZO; CITRUS-AURANTIUM; EMBRYOIDS; PONCIRUS-TRIFOLIATA;

D O I：

10.1007/BF00044262

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Complete plants were regenerated from callus derived embryo culture of Citrus aurantium, Poncirus trifoliata and of Carrizo citrange (C. sinensis x Poncirus trifoliata) via somatic embryogenesis and organogenesis. Isolated embryoids were differentiated on medium containing 1000 to 1500 mg l-1 malt extract. Both embryoids and shoots were obtained by the use of plant growth regulators: NAA and BAP. The highest frequency was obtained with 1 mg l-1 NAA + 5 mg l-1 BAP in Poncirus trifoliata and citrange but with 1 mg l-1 NAA + 10 mg l-1 BAP in C. aurantium. Rooting was promoted by a supplement of 1 mg l-1 GA3 or 1 mg l-1 NAA. GA3 enhanced stem elongation and rooting in embryoids and NAA stimulated adventitous root formation.

引用

页码：29 / 34

页数：6

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