TARGETED BREAKAGE OF A HUMAN-CHROMOSOME MEDIATED BY CLONED HUMAN TELOMERIC DNA

被引：67

作者：

ITZHAKI, JE ^{[1
]}

BARNETT, MA ^{[1
]}

MACCARTHY, AB ^{[1
]}

BUCKLE, VJ ^{[1
]}

BROWN, WRA ^{[1
]}

PORTER, ACG ^{[1
]}

机构：

[1] JOHN RADCLIFFE HOSP,INST MOLEC MED,OXFORD OX3 9DU,ENGLAND

来源：

NATURE GENETICS | 1992年 / 2卷 / 04期

关键词：

D O I：

10.1038/ng1292-283

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Novel approaches to the structural and functional analysis of mammalian chromosomes would be possible if the gross structure of the chromosomes in living cells could be engineered. Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled modifications can be made by the integration of cloned human telomeric DNA. We describe here the combined use of gene targeting and telomere-mediated chromosome breakage to generate a defined truncation of a human chromosome. Telomeric DNA was targeted to the 6-16 gene on the short arm of chromosome 1 in a human cell line. Molecular and cytogenetic analyses showed that, of eight targeted clones that were isolated, one clone had the predicted truncation of chromosome 1.

引用

页码：283 / 287

页数：5

共 21 条

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