BARRIER PROPERTIES OF TESTIS MICROVESSELS

被引:111
作者
HOLASH, JA
HARIK, SI
PERRY, G
STEWART, PA
机构
[1] UNIV HOSP CLEVELAND, DEPT NEUROL, CLEVELAND, OH 44106 USA
[2] UNIV HOSP CLEVELAND, DEPT PATHOL, CLEVELAND, OH 44106 USA
[3] CASE WESTERN RESERVE UNIV, SCH MED, CLEVELAND, OH 44106 USA
关键词
D O I
10.1073/pnas.90.23.11069
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The blood-testis barrier is believed to be constituted by tight junctions between Sertoli cells in seminiferous tubules and possibly by myoid cells that encircle these tubules. We now show that testis microvessels are endowed with several markers of barrier properties of brain microvessels, such as the glucose transporter, P-glycoprotein, and gamma-glutamyl transpeptidase. Quantitative EM studies show that the endothelium in testis, as in brain, is continuous and has long junctional profiles and few vesicles. However, a small proportion of testis capillaries have expansions in their junctional clefts suggestive of patent paracellular channels, which may explain their higher permeability. Because barrier features are thought to be induced and/or maintained in brain microvessels by astrocytes, we assessed whether astrocyte-like cells exist in the testis. We found that the intertubular Leydig cells, adjacent to microvessels, express the astrocyte markers: glial fibrillary acidic protein, glutamine synthetase, and S-100 protein. We suggest that the testis endothelium contributes to the blood-testis barrier and that these endothelial barrier features are influenced by Leydig cells. We believe that the endothelial and the epithelial (Sertoli) components of the blood-testis barrier are ''in series'' and complement each other in achieving a stable milieu for spermatogenesis.
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页码:11069 / 11073
页数:5
相关论文
共 53 条
[1]   LOCALIZATION OF GLIAL FIBRILLARY ACIDIC PROTEIN IN ASTROCYTES BY IMMUNOFLUORESCENCE [J].
BIGNAMI, A ;
ENG, LF ;
DAHL, D ;
UYEDA, CT .
BRAIN RESEARCH, 1972, 43 (02) :429-&
[2]   JUNCTIONS BETWEEN INTIMATELY APPOSED CELL MEMBRANES IN VERTEBRATE BRAIN [J].
BRIGHTMA.MW ;
REESE, TS .
JOURNAL OF CELL BIOLOGY, 1969, 40 (03) :648-+
[3]   THE 3-DIMENSIONAL ORGANIZATION OF TIGHT JUNCTIONS IN A CAPILLARY ENDOTHELIUM REVEALED BY SERIAL-SECTION ELECTRON-MICROSCOPY [J].
BUNDGAARD, M .
JOURNAL OF ULTRASTRUCTURE RESEARCH, 1984, 88 (01) :1-17
[4]  
CASLEYSMITH JR, 1981, MICROCIRCULATION, V1, P79
[5]   A QUANTITATIVE ASSESSMENT OF MICROVESSEL ULTRASTRUCTURE IN C-6 ASTROCYTOMA SPHEROIDS TRANSPLANTED TO BRAIN AND TO MUSCLE [J].
COOMBER, BL ;
STEWART, PA ;
HAYAKAWA, EM ;
FARRELL, CL ;
DELMAESTRO, RF .
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1988, 47 (01) :29-40
[6]   MULTIDRUG-RESISTANCE GENE (P-GLYCOPROTEIN) IS EXPRESSED BY ENDOTHELIAL-CELLS AT BLOOD-BRAIN BARRIER SITES [J].
CORDONCARDO, C ;
OBRIEN, JP ;
CASALS, D ;
RITTMANGRAUER, L ;
BIEDLER, JL ;
MELAMED, MR ;
BERTINO, JR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (02) :695-698
[7]   EXPRESSION OF THE MULTIDRUG RESISTANCE GENE-PRODUCT (P-GLYCOPROTEIN) IN HUMAN NORMAL AND TUMOR-TISSUES [J].
CORDONCARDO, C ;
OBRIEN, JP ;
BOCCIA, J ;
CASALS, D ;
BERTINO, JR ;
MELAMED, MR .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1990, 38 (09) :1277-1287
[8]  
CRONE C, 1985, CLIN INVEST MED, V8, P89
[9]   FACILITATED TRANSFER OF GLUCOSE FROM BLOOD INTO BRAIN TISSUE [J].
CRONE, C .
JOURNAL OF PHYSIOLOGY-LONDON, 1965, 181 (01) :103-&
[10]  
DERMIETZEL R, 1991, INT REV CYTOL, V127, P57