SWITCHING CAPACITY OF FC-EPSILON-RII-POSITIVE AND FC-EPSILON-RII-NEGATIVE MURINE B-CELLS

In previous studies, our laboratory demonstrated the utility of the low affinity IgE Fc receptor (FcepsilonRII) in delineating a number of murine B cell subsets. In the spleen, the FcepsilonRII is expressed on mature conventional B cells but is absent on marginal zone B cells. In the peritoneal cavity, the receptor is present on all conventional B cells, but is not expressed on fresh peritoneal Ly1/sister B cells. The studies in this report compared the ability of these B cell poputations to isotype switch. Using a lipopolysaccharide (LPS)- and interleukin (IL)-4-driven system, sort-purified FcepsilonRII- positive and -negative B cells from peritoneum and spleen were tested for switching to IgG1, IgE, and IgA. The results demonstrated that regardless of their source, FcepsilonRII+ B cells produced significant levels of IgG1 and IgE. Similar results were obtained with FcepsilonRII- (marginal zone) B cells obtained from spleen. In contrast, FcepsilonRII- (Ly1/sister) peritoneal B cells were found to produce IgG1 and IgA, but were incapable, of secreting significant levels of IgE. Further studies tested for LPS and IL-4-induced expression of FcepsilonRII and Thy1 on the various B cell populations. These experiments demonstrated the induction of the FcepsilonRII on all B cells, regardless of their initial resting levels. Additionally, Thy1 was found to be induced only on those B cell subsets capable of producing IgE. Taken together, the results demonstrate a correlation between IgE secretion and Thy1 expression, and no apparent correlation between the presence of the FcepsilonRII and isotype commitment.