IMMUNOCHEMISTRY OF SOME ARTIFICIAL HUMAN HEMOGLOBINS

The preparation of globin complexes with zinc metaloporphyrin, protoporphyrin IX and ferriheme dipyrid-4-ylpropyl ester is described and their physicochemical properties investigated. Reconstitution of hemoglobin from globin and resynthesized heme (i.e. from ferric iron and protoporphyrin IX) gave a preparation having conformational parameters and immunochemical behavior identical with those of the native protein. Hemoglobin derivative prepared with protoporphyrin IX showed a great increase in the Stokes radius which was accompanied by an enhancement of the antigenic reactivity of the derivative (relative to native hemoglobin). Similar increase in antigenic reactivity was obtained with hemoglobin containing heme dipyrid-4-ylpropyl ester, altough only relative slight changes in conformational paramaters were observed. The Stokes radius of the hemoglobin derivative prepared with zinc metalloporphyrin was greatly increased and reacted poorly with antisera to hemoglobin. © 1969.