PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF A VIRUS-SPECIFIC REVERSE-TRANSCRIPTASE FROM HUMAN OSTEOSARCOMA TISSUE

被引：10

作者：

EBENER, U ^{[1
]}

WELTE, K ^{[1
]}

CHANDRA, P ^{[1
]}

机构：

[1] JOHANN WOLFGANG GOETHE UNIV KLINIKUM, GUSTAV EMBDEN ZENTRUM BIOL CHEM, MOLEK BIOL ABT, D-6000 FRANKFURT 70, GERMANY

来源：

CANCER LETTERS | 1979年 / 7卷 / 04期

关键词：

D O I：

10.1016/S0304-3835(79)80078-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A RNA-dependent DNA polymerase (RTase) was purified from human osteosarcoma tissus by successive column chromatography of the micro-somal fraction on DEAE-cellulose (DE-23 and DE-52) and phosphocellulose. The purified enzyme has a molecular weight of about 68,000, a pH optimum of 8.1, a Mg2+ optimum of 0.8 mM, Mn2+ optimum of 1.0 mM and a KCl optimum of 60 mM. The enzyme transcribes (rA)n · (dT)12, (rC)n · (dG)12-18 and (2-O-methyl C)n · (dG)18, but is unable to transcribe (dA)n · (dT)10. The enzyme has no catalytic activity in the presence of oligodeoxynucleotide initiators alone, indicating the absence of terminal deoxynucleotidyl transferase. The purified enzyme is able to transcribe the heteropolymeric regions of a 70S RNA from R(Mu)LV. The presented data support the presence of a RNA-dependent DNA polymerase in human osteosarcoma tissue with biochemical properties, resembling those of C-type RNA tumor viruses. © 1979 Elsevier/North-Holland Scientific Publishers Ltd., All rights reserved.

引用

页码：179 / 188

页数：10

共 27 条

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[2]

2-F

[3]

BOWEN JM, 1973, AM J CLIN PATHOL, V60, P88

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