PHAGES-T7 IN BIOLOGICAL UV DOSE MEASUREMENT

被引:62
作者
RONTO, G
GASPAR, S
BERCES, A
机构
[1] Institute of Biophysics, Semmelweis University of Medicine, Budapest
基金
匈牙利科学研究基金会;
关键词
UV DOSIMETRY; PHAGE DOSIMETER; MINIMAL ERYTHEMA DOSE; SPECTRAL SENSITIVITY; ACTION SPECTRA;
D O I
10.1016/1011-1344(92)85030-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An experimental method complete with theoretical considerations is presented for the measurement of different biological UV doses. The method is based on the high sensitivity of phage T7 activity to UV light. A precisely determined T7 inactivation action spectrum is presented over a wide optical range (240-514 nm). Using the T7 spectral sensitivity in relation to the minimal erythema dose (MED) and the effective spectral irradiance from solar radiation for the MED, an example is given to determine the MED value based on the measurement of T7 inactivation for a given case. The advantages and applicability of the method are discussed.
引用
收藏
页码:285 / 294
页数:10
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