SPECTROPHOTOMETRIC AND FLUOROMETRIC-DETERMINATION OF CATECHOLAMINES

被引:39
作者
SALEM, FB
机构
[1] Faculty of Pharmacy, Mansoura University, Mansoura
关键词
SPECTROPHOTOMETRIC; SPECTROFLUOROMETRIC; CATECHOLAMINES; O-PHENYLENEDIAMINE DIHYDROCHLORIDE; 1,2-DIAMINOANTHRACENE AND 2-AMINOPHENOL;
D O I
10.1080/00032719308017385
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Spectrophotometric and spectrofluorometric methods have been described for determination of catecholamines in pharmaceutical formulations. These depend upon measuring the intensity of the orange colour produced on reacting catecholamines with the ethanolic solution of o-phenylenediamine dihydrochloride at 490 nm and at 560, 410 nm emission maxima with 1,2-diaminoanthrancene and 2-aminophenol. The absorbance obeys Beer's law in the concentration range of 0.092 to 2.48 mg of catechol amines spectrophotometrically from 4.5 to 240 mug spectrofluorometrically.
引用
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页码:281 / 294
页数:14
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