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CLONING AND EXPRESSION ANALYSIS OF A CYTOCHROME P-450(11-BETA) CDNA IN SHEEP

被引：20
作者：
BOON, WC
ROCHE, PJ
HAMMOND, VE
JEYASEELAN, K
CRAWFORD, RJ
COGHLAN, JP
机构：
[1] UNIV MELBOURNE,HOWARD FLOREY INST EXPTL PHYSIOL & MED,PARKVILLE,VIC 3052,AUSTRALIA
[2] NATL UNIV SINGAPORE,DEPT BIOCHEM,SINGAPORE 0511,SINGAPORE
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1260卷 / 01期
基金：
英国医学研究理事会;
关键词：
CYTOCHROME P-45011-BETA; STEROID; 11-BETA-HYDROXYLASE; NUCLEOTIDE SEQUENCE; ADRENAL CORTEX; (SHEEP);
D O I：
10.1016/0167-4781(94)00190-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A full length ovine steroid 11 beta-hydroxylase (cytochrome P-450(11 beta)) cDNA crane from a sheep adrenal cortex cDNA library was isolated. Sequence analysis indicates that this cDNA clone resembles bovine P-450(11 beta) cDNA(95% nucleotide sequence homology) more closely than rat P-450(11 beta) cDNA (69% nucleotide sequence homology). Although the levels of nucleotide sequence homology of this cDNA clone to the rat P-450(11 beta) cDNA and the rat P-450(aldo) cDNA are similar, the putative amino acid sequence shows a closer resemblance to rat P-450(aldo) protein. Northern blot analysis shows that there are three sizes of transcript and they are expressed throughout the adrenal cortex.
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收藏
页码：109 / 112
页数：4
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