RAPID FRACTIONATION OF HUMAN HIGH-DENSITY APOLIPOPROTEINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A simple and rapid fractionation procedure (30 min) was developed for the isolation of the major apoproteins from human serum high density lipoproteins by molecular sieving in a high performance liquid chromatographic column. ApoA-I, apoA-II and the C peptides are quantitatively resolved up to a protein load of 3 mg. The technique was also successfully applied to the final purification of A apoproteins which had been isolated by conventional chromatogrpahic procedures and as a sensitive analytical tool for assessing apoprotein purity.