APPLICATION OF THE RANDOM AMPLIFIED POLYMORPHIC DNA USING THE POLYMERASE CHAIN-REACTION FOR EFFICIENT ELIMINATION OF DUPLICATE STRAINS IN MICROBIAL SCREENING .2. ACTINOMYCETES

被引：13

作者：

ANZAI, Y ^{[1
]}

OKUDA, T ^{[1
]}

WATANABE, J ^{[1
]}

机构：

[1] NIPPON ROCHE RES CTR,DEPT MICROBIOL & TAXON,KAMAKURA,KANAGAWA 247,JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1994年 / 47卷 / 02期

关键词：

D O I：

10.7164/antibiotics.47.183

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We evaluated the random amplified polymorphic DNA (RAPD) method using Streptomyces lavendulae and Streptomyces virginiae strains to eliminate duplicate actinomycete strains in out microbial screening program. The RAPD data were compared with phenotypic characteristics, DNA relatedness, HPLC analysis of metabolites and low-frequency restriction fragment analysis by pulsed-field gel electrophoresis. These results were consistent with each other. Therefore, we conclude that RAPD is a simple, efficient, and reliable method for the selection of actinomycete strains.

引用

页码：183 / 193

页数：11

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