OBSERVATION OF THE HELICAL STRUCTURE OF THE BACTERIAL POLYSACCHARIDE ACETAN BY ATOMIC-FORCE MICROSCOPY

被引：56

作者：

KIRBY, AR ^{[1
]}

GUNNING, AP ^{[1
]}

MORRIS, VJ ^{[1
]}

RIDOUT, MJ ^{[1
]}

机构：

[1] INST FOOD RES, NORWICH NR4 7UA, NORFOLK, ENGLAND

来源：

BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 01期

关键词：

D O I：

10.1016/S0006-3495(95)80195-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A method has been developed that has been found to give reproducible images of uncoated polysaccharides by Atomic Force Microscopy (AFM). Aqueous solutions of the polysaccharide are deposited as drops onto freshly cleaved mica surfaces, air dried, and then imaged under butanol. The method has been used to obtain images of the bacterial polysaccharide acetan. In regions within the deposited sample, where the molecules are aligned side-by-side, it has been possible to observe a periodic structure along the polysaccharide chain, attributable to the helical structure of acetan.

引用

页码：360 / 363

页数：4

共 35 条

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