CELL-SPECIFIC ANTIGENS IN CHICKEN ERYTHROID NUCLEI - SPECIES SPECIFICITY

被引:17
作者
KRAJEWSKA, WM
BRIGGS, RC
HNILICA, LS
机构
[1] VANDERBILT UNIV, SCH MED, DEPT BIOCHEM, NASHVILLE, TN 37232 USA
[2] VANDERBILT UNIV, SCH MED, AB HANCOCK JR MEM LAB, NASHVILLE, TN 37232 USA
关键词
D O I
10.1021/bi00592a031
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Antisera raised to dehistonized chicken reticulocyte chromatin were tested for their cell and species specificity. Quantitative microcomplement fixation and immunohisto-chemical localization revealed the presence in chromatin of erythroid cell-specific nonhistone protein antigen(s). The antigenic specificity was shown to depend on the association of the antigenic protein(s) with deoxyribonucleic acid (DNA). Although the antisera were exceptionally cell specific, they cross-reacted with erythroid cells of other avian species. The extent of cross-reactivity was found to approximate the phy-logenetic distances of the tested avian species. Erythroid cells from fish and amphibians were not reactive. Reconstitution experiments of partially purified chicken reticulocyte chromosomal nonhistone protein antigens with DNAs isolated from several vertebrate species showed that the species specificity of the antigenic complexes is determined principally by the species origin of the nonhistone proteins. Our results show that a cell-specific chromosomal nonhistone protein(s) has undergone evolutionary change and the relative immunological differences observed are consistent with the accepted phylo-genetic distances of the species examined. © 1979, American Chemical Society. All rights reserved.
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页码:5720 / 5725
页数:6
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