FAST AND SIMPLE METHOD FOR SEQUENCING DNA CLONED IN THE SINGLE-STRANDED BACTERIOPHAGE-M13

被引：231

作者：

SCHREIER, PH ^{[1
]}

CORTESE, R ^{[1
]}

机构：

[1] UNIV COLOGNE,INST GENET,D-5000 COLOGNE 41,FED REP GER

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1979年 / 129卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0022-2836(79)90068-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chain termination DNA sequencing procedure of Sanger et al. (1977) requires single-stranded DNA as template. M13 phage DNA exists as a single strand and therefore every DNA sequence cloned in M13 can be easily obtained in this form. Here we show that M13 single-stranded DNA pure enough to be used as a template for sequence determination can be prepared by simple centrifugation of the phage particle and extraction with phenol. © 1979.

引用

页码：169 / 172

页数：4

共 10 条

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