ANTIGENIC DETERMINANTS AND SPECIFICITY OF ANTISERA AGAINST ACIDOPHILIC BACTERIA

被引：5

作者：

KOPPE, B ^{[1
]}

HARMS, H ^{[1
]}

机构：

[1] UNIV HAMBURG, INST ALLGEMEINE BOT, MIKROBIOL ABT, OHNHORSTSTR 18, D-22609 HAMBURG, GERMANY

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1994年 / 10卷 / 02期

关键词：

ANTIGEN; IMMUNOFLUORESCENCE; LEPTOSPIRILLUM-FERROOXIDANS; LIPOPOLYSACCHARIDE; THIOBACILLUS SPP;

D O I：

10.1007/BF00360877

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyclonal antisera, produced against whole cells of Thiobacillus thiooxidans, T. ferrooxidans and Leptospirillum ferrooxidans, gave highly specific reactions when cross-reacted with 23 strains of acidophilic bacteria using an immunofluorescence (IF) staining technique. Strains of identical serotype exhibited maximum cross-reaction whereas strains of different serotype reacted only weakly. Lipopolysaccharides (LPS) examined by SDS-PAGE showed different, serotype-specific migration patterns indicating their rough or smooth character. LPS patterns may therefore be used for serological classification of acidophilic bacteria. Surface antigens of four strains were identified by immunoblot staining; LPS and some proteins were antigenic determinants with LPS the most specific.

引用

页码：154 / 158

页数：5

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