EVIDENCE FOR THE PRESENCE OF BETA-3-ADRENERGIC RECEPTOR MESSENGER-RNA IN THE HUMAN BRAIN

被引：54

作者：

RODRIGUEZ, M

CARILLON, C

COQUEREL, A

LEFUR, G

FERRARA, P

CAPUT, D

SHIRE, D

机构：

[1] SANOFI RECH, F-71676 LABEGE, FRANCE

[2] HOP CHARLES NICOLLE, RADIOANAL & HORMONOL LAB, F-76031 ROUEN, FRANCE

来源：

MOLECULAR BRAIN RESEARCH | 1995年 / 29卷 / 02期

关键词：

ADRENERGIC BETA-3 RECEPTOR; HUMAN BRAIN; PCR; QUANTITATIVE PCR; CLONING; SEQUENCE ANALYSIS;

D O I：

10.1016/0169-328X(94)00274-I

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The beta 3-adrenergic receptor (AR) is widely distributed in peripheral tissues, but up to now it has not been detected in the central nervous system. By using the polymerase chain reaction (PCR) technique, we found the beta 3-AR mRNA to be present in all the regions of the human brain we investigated. The quantities found were very low compared to those of the beta 1-AR and beta 2-AR mRNAs, being hardly detectable in adult brain. In contrast, the brain of very young infants contained about 100 times more beta 3-AR mRNA than the adult brain, whereas the amounts of beta 1-AR and beta 2-AR transcripts were essentially the same. In addition, using PCR we have cloned a central beta 3-AR coding region from a human frontal cortex cDNA library and have found it to be identical to the corresponding peripheral sequence.

引用

页码：369 / 375

页数：7

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