RAPID AND SPECIFIC FLUORESCENT ACTIVITY STAINING PROCEDURE FOR TRANSAMIDATING ENZYMES

被引：27

作者：

STENBERG, P

STENFLO, J

机构：

[1] Department of Clinical Chemistry, The General Hospital

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 93卷 / 02期

关键词：

D O I：

10.1016/S0003-2697(79)80177-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, sensitive, and specific procedure has been developed for detection of transsamidatingenzymes (transglutaminase, e.g., factor XIII) after agarose gel electrophoresis. The technique is based on the transamidase-catalyzed incorporation of the fluorescent monodansylthiacadaverine into casein. The high sensitivity enables detection and characterization of transamidases in blood plasma, platelet, and red blood cell lysate and tissue extracts. The technique can also be combined with crossed immunoelectrophoresis. © 1979 Academic Press, Inc.

引用

页码：445 / 452

页数：8

共 30 条

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