MATRIX-ASSISTED INFRARED-LASER (2.94-MU-M) DESORPTION IONIZATION MASS-SPECTROMETRY OF LARGE BIOMOLECULES

被引：161

作者：

OVERBERG, A ^{[1
]}

KARAS, M ^{[1
]}

BAHR, U ^{[1
]}

KAUFMANN, R ^{[1
]}

HILLENKAMP, F ^{[1
]}

机构：

[1] UNIV MUNSTER,INST MED PHYS,HUFFERSTR 68,W-4400 MUNSTER,GERMANY

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 08期

关键词：

D O I：

10.1002/rcm.1290040808

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The matrix‐assisted desorption/ionization of large biomolecules with a wavelength of 2.94 μm in the infrared is reported. A mechanically Q‐switched Erbium‐YAG laser with a pulse width of ca. 200 ns was used for the experiments. A large variety of matrices have been used successfully, among them all matrices found useful for ultraviolet desorption and, in addition, carboxylic acids, glycerol and urea. Desorption of molecules at this wavelength and caffeic acid as a matrix results in ions with up to 13 charges per ion. Copyright © 1990 John Wiley & Sons Limited

引用

页码：293 / 296

页数：4

共 19 条

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[4] Karas M., Hillenkamp F., Int. J. Mass Spectrom. Ion Proc., 92, (1989)
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[7] Hillenkamp F., Karas M., Ingendoh A., Stahl B., Biological Mass Spectrometry
[8] Beavis R.C., Chait B.T., Rapid Commun. Mass Spectrom., 3, (1989)
[9] Beavis R.C., Chait B.T., Rapid Commun. Mass Spectrom., 3, (1989)
[10] Beavis R.C., Chait B.T., Rapid Commun. Mass Spectrom., 3, (1989)

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