CONTINUOUS CONVERSION TO OPTICALLY PURE L-METHIONINE FROM D-ENANTIOMER CONTAMINATED PREPARATIONS BY AN IMMOBILIZED ENZYME MEMBRANE REACTOR

被引：26

作者：

NAKAJIMA, N

CONRAD, D

SUMI, H

SUZUKI, K

ESAKI, N

WANDREY, C

SODA, K

机构：

[1] OKAYAMA PREFECTURAL JR COLL,DEPT FOOD & NUTR,OKAYAMA 700,JAPAN

[2] KYOTO UNIV,INST CHEM RES,MICROBIAL BIOCHEM LAB,UJI,KYOTO 611,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1990年 / 70卷 / 05期

关键词：

D O I：

10.1016/0922-338X(90)90142-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Continuous conversion to optically pure l-methionine from preparations contaminated by a small amount of the d-enantiomer (0.5-1.0%) has been developed with an enzyme membrane reactor system (slurry reactor) containing immobilized d-amino acid oxidase, catalase, and FAD. The optimal pH of the reaction system was from pH 7.0-8.0, and the optimum temperature was 25°C. The average conversion and the space-time yield were > 99% e.e. and nearly 90 g/l·d, respectively with operation of the reactor for 7 d. © 1990.

引用

页码：322 / 325

页数：4

共 9 条

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[2] ASSAY OF CATALASES AND PEROXIDASES [J].