MOLECULAR-WEIGHT ANALYSIS OF ISOPENICILLIN-N SYNTHASE BY ELECTROSPRAY MASS-SPECTROMETRY

被引：13

作者：

APLIN, RT

BALDWIN, JE

FUJISHIMA, Y

SCHOFIELD, CJ

GREEN, BN

JARVIS, SA

机构：

[1] DYSON PERRINS LAB,S PARKS RD,OXFORD OX1 3QY,ENGLAND

[2] OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND

[3] VG MASS LAB LTD,ALTRINCHAM WA14 5RZ,CHESHIRE,ENGLAND

来源：

FEBS LETTERS | 1990年 / 264卷 / 02期

关键词：

Cephalosporium acremonium; Electrospray mass spectrometry; Isopenicillin N synthase; Molecular weight; Penicillin biosynthesis;

D O I：

10.1016/0014-5793(90)80251-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of electrospray mass spectrometry as a tool in analytical biochemistry was illustrated by determination of the molecular weights of wildtype and recombinant isopenicillin N synthase (IPNS). The molecular weight of recombinant IPNS produced using an expression system which generated soluble protein was found to be between 38 364 and 38 376 Da, ca 60 mass units higher than that of the wildtype material, consistent with the presence of an additional N-terminal glycine in the former. Observed molecular weights were all ca 70 Da higher than that calculated from sequence information, consistent with the complexion of a partially hydrated iron atom to the enzyme during analysis. © 1990.

引用

页码：215 / 217

页数：3

共 15 条

[1] N-TERMINAL AMINO-ACID SEQUENCE AND SOME PROPERTIES OF ISOPENICILLIN-N SYNTHETASE FROM CEPHALOSPORIUM-ACREMONIUM
BALDWIN, JE
GAGNON, J
TING, HH
[J]. FEBS LETTERS, 1985, 188 (02) : 253 - 256
[2] THE BIOSYNTHESIS OF PENICILLINS AND CEPHALOSPORINS
BALDWIN, JE
ABRAHAM, E
[J]. NATURAL PRODUCT REPORTS, 1988, 5 (02) : 129 - 145
[3] PURIFICATION AND CHARACTERIZATION OF CLONED ISOPENICILLIN-N SYNTHETASE
BALDWIN, JE
KILLIN, SJ
PRATT, AJ
SUTHERLAND, JD
TURNER, NJ
CRABBE, MJC
ABRAHAM, EP
WILLIS, AC
[J]. JOURNAL OF ANTIBIOTICS, 1987, 40 (05) : 652 - 659
[4] BALDWIN JE, IN PRESS FEMS LETT
[5] BALDWIN JE, 1985, RECENT ADV CHEM BETA, P65
[6] CHEN VJ, 1989, J BIOL CHEM, V264, P21677
[7] Covey T R, 1988, Rapid Commun Mass Spectrom, V2, P249, DOI 10.1002/rcm.1290021111
[8] ANTI-TERMINATION AND TERMINATION FUNCTIONS OF THE CLONED NUTL, N AND TL1 MODULES OF COLIPHAGE LAMBDA
DRAHOS, D
SZYBALSKI, W
[J]. GENE, 1981, 16 (1-3) : 261 - 274
[9] EDMONDS CG, 1989, 37TH P ASMS C MASS S, P844
[10] JARVIS SA, 1989, 37TH P ASMS C MASS S, P1017

← 1 2 →