NOVEL MONOMERIC LUCIFERASE ENZYMES AS TOOLS TO STUDY PLANT GENE-REGULATION INVIVO

被引：10

作者：

OLSSON, O ^{[1
]}

NILSSON, O ^{[1
]}

KONCZ, C ^{[1
]}

机构：

[1] MAX PLANCK INST ZUCHTUNGSFORSCH,W-5000 COLOGNE 30,GERMANY

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1990年 / 5卷 / 02期

关键词：

D O I：

10.1002/bio.1170050203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Taking advantage of a specially constructed vector, luciferase LuxA and LuxB subunits were connected in frame to different amino acid linkers to reproduce a series of monomeric luciferase enzymes. A comparison of their activities in E. coli cells demonstrated that the length of the linkers positively affected activity. One luciferase fusion gene was expressed in plant cells, and we showed that this gene activity could be monitored directly without destructive sampling.

引用

页码：79 / 87

页数：9

共 24 条

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