Thyroid Hormone Up-regulates NGFI-A Gene Expression in Rat Brain during Development

被引:54
作者
PIPAON, C
SANTOS, A
PEREZCASTILLO, A
机构
[1] CSIC, INST INVEST BIOMED, E-28029 MADRID, SPAIN
[2] UNIV COMPLUTENSE MADRID, FAC MED,DEPT BIOQUIM & BIOL MOLEC, E-28040 MADRID, SPAIN
关键词
D O I
10.1016/S0021-9258(18)48449-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
NGFI-A is an immediate-early response gene induced by signals that initiate growth and differentiation. Its mRNA encodes a sequence-specific transcriptional activator possibly implicated in the control of brain developmental processes. Due to the essential role of thyroid hormone for a correct brain development, we have now investigated the possible regulation by 3,5,3'-triiodo-L-thyronine (T3) of NGFI-A gene expression during maturation of the central nervous system. Our results indicate that expression of mRNA encoding NGFI-A transcription factor is about 8-fold decreased in the brain of neonatal hypothyroid rats. No changes were seen when hypothyroidism was induced in adult life. T3 treatment increased NGFI-A mRNA within 1 h, suggesting that thyroid hormone effect is likely to be a direct one. These data indicate a strong regulation by thyroid hormone of the expression of the growth factor inducible gene NGFI-A during brain development, making this gene a suitable model to study T3 action in the early developing nervous system.
引用
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页码:21 / 23
页数:3
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